Heather Watson has been beaten in the US Open first round for the eighth successive year, losing 6-1 3-6 6-3 to Ekaterina Makarova in New York.
Watson, ranked 116th, won five games in a row from 3-1 down in the second set but a single break in the decider was enough to give the Russian victory.
The British number three, 26, won the Flushing Meadows junior title in 2009 but is yet to win a main-draw match.
She converted only two of seven break-point opportunities against Makarova.
World number 49 Makarova, a 2014 semi-finalist, took four of her six break-point chances.